Team:IBB Pune/Parts

From 2009.igem.org

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{{Team:IBB_Pune/header}}
{{Team:IBB_Pune/header}}
{{Team:IBB_Pune/menu}}
{{Team:IBB_Pune/menu}}
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<br><br>
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<p><span style="font-weight:bold; font-size:200%; color:#000000;">Parts shipped to the registry</span></p>
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[[Image:Truck.jpg|center| "width: 1400 px"]]
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== Parts Submitted to the Registry ==
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{|class="wikitable sortable" border="1"
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1) <html>
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|- style="background: #339933; text-align: center;"
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<a href="http://partsregistry.org/Part:BBa_K233001"> Part: BBa_K233001</a>
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|width=15| '''Sr No.'''
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</html> Still in the design stage. We will try to create this DNA part in our lab.
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|width=85| '''BioBrick Name'''
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|width=160| '''Part Name'''
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2) <html>
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|width=85| '''TYPE'''
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<a href="http://partsregistry.org/Part:BBa_K233002"> Part: BBa_K233002</a>
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|width=980| '''Description'''
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</html> Still in the design stage. We will try to create this DNA part in our lab.
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|width=95| '''Backbone'''
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|width=50| '''LENGTH (bp)'''
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3) <html>
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|width=1| '''SEQUENCED'''
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<a href="http://partsregistry.org/Part:BBa_K233003"> Part: BBa_K233003</a>
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|- style="background:#66ffcc; color:black; text-align: center"
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</html> Still in the design stage. We will try to create this DNA part in our lab.
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| 1
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| [http://partsregistry.org/Part:BBa_K233306 BBa_K233306] 
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4) <html>
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| YcdB
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<a href="http://partsregistry.org/Part:BBa_K233004"> Part: BBa_K233004</a>
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| Signalling
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</html> We have successfully obtained and shipped this part. It is the lactose operator induced by lactose or IPTG. This part is an  integral cog in our AND gate.
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| YcdB - This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT)
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| [http://partsregistry.org/Part:BBa_K233324 BBa_K233324]
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5) <html>
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| 123
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| Yes
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|- style="background:#66ffcc; color:black; text-align: center"
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| 2
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233004 BBa_K233004] 
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| lac operator
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| regulatory
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| Lac operator.
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| [http://partsregistry.org/Part:BBa_K233324 BBa_K233324]
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| 30
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| Yes
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|- style="background:#66ffcc; color:black; text-align: center"
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| 3
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233307 BBa_K233307] 
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| TorA
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| Signalling
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| TorA- This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT)
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| [http://partsregistry.org/Part:BBa_K233324 BBa_K233324]
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| 139
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| Yes
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|- style="background:#66ffcc; color:black; text-align: center"
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| 4
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233008 BBa_K233008] 
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| TorA-GFP
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| Signalling
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| This construct is an intermediate in the production of an exportable GFP.
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| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
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| 865
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| -
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|- style="background:#66ffcc; color:black; text-align: center"
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| 5
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233309 BBa_K233309]  
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| YcdB-GFP
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| Signalling
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| This is an intermediate which can be used for constructing a module which can be used to produce an exportable GFP
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| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
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| 849
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| -
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|- style="background:#66ffcc; color:black; text-align: center"
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| 6
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233310 BBa_K233310] 
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| constitutive GFP device
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| Reporter
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| Promoter-RBS-GFP- constitutive green fluorescent protein device
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| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
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| 800
 +
| -
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|- style="background:#66ffcc; color:black; text-align: center"
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| 7
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233311 BBa_K233311] 
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| Protein(GFP) secretion device
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| Device
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| This device codes for an exportable GFP
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| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
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| 945
 +
| -
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|- style="background:#66ffcc; color:black; text-align: center"
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| 8
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233312 BBa_K233312] 
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| GFP secretion device using Ycdb
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| Device
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| This device codes for an exportable GFP
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| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
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| 929
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| -
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|- style="background:#66ffcc; color:black; text-align: center"
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| 9
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233313 BBa_K233313] 
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| ahl induced ahl producer
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| Composite
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| construct designed to produce AHL (a quorum sensing molecule) in response to environmental AHL
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| [http://partsregistry.org/Part:pSB1AK3 pSB1AK3]
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| 725
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| -
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|- style="background:#66ffcc; color:black; text-align: center"
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| 10
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233314 BBa_K233314] 
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| constitutive luxR production device
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| Generator
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| This part allows constitutive production of the LuxR protein which binds to AHL and the LuxR-AHL complex activates the pLuxR promoter.
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| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
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| 881
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| -
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|- style="background:#66ffcc; color:black; text-align: center"
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| 11
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| [http://partsregistry.org/wiki/index.php?title=Part:BBa_K233315 BBa_K233315] 
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| constitutive LuxR generating device
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| Generator
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|This part allows constitutive production of the LuxR protein
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| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
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| 824
 +
| -
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|- style="background:#66ffcc; color:black; text-align: center"
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|12
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K233316 BBa_K233316]
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|Constitutive promoter lac operator
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|Device
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|This part can be used to calibrate the working of the lac operator
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| [http://partsregistry.org/Part:pSB1A2 pSB1A2]
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|73
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| -
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|- style="background:#66ffcc; color:black; text-align: center"
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|13
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K233317 BBa_K233317]
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|rfp measurement device
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|measurement
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|This part can be used to calibrate the working of BBa_J13002
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|[http://partsregistry.org/Part:pSB1A2 pSB1A2]
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|912
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| -
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|-style="background:#66ffcc; color:black; text-align: center"
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| 14
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| [http://partsregistry.org/Part:BBa_K233003 BBa_K233003] 
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| AND Gate 1
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| Regulatory
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| This is an AND Gate, responsive to two external inputs, Lactose and AHL.
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| [http://partsregistry.org/Part:pSB1C3 pSB1C3]
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| 94
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| -
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|}
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<br>
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<p><span style="font-weight:bold; font-size:200%; color:#000000;">Parts designed by us</span></p><br>
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{|class="wikitable sortable" border="1"
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|- style="background: #339933; Align: center;"
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|width=100| '''Sr No.'''
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|width=200| '''BioBrick Name'''
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|width=400| '''Part Name'''
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|WIDTH=400|'''Description'''
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|-
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|- style="background:#66ffcc; color:black; text-align: center"
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|1
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|<html> <a href="http://partsregistry.org/Part:BBa_K233002"> Part: BBa_K233002</a> </html>  
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|Snowdrift 2 , using YcdB.  
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|A module to secrete B-galactosidase using the TAT-export Pathway
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|-
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|- style="background:#66ffcc; color:black; text-align: center"
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|2
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|<html> <a href="http://partsregistry.org/Part:BBa_K233001"> Part: BBa_K233001</a>
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</html>
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|Snowdrift 1 , using TorA
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| A module to secrete B-galactosidase using the TAT-export Pathway
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|-
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|- style="background:#66ffcc; color:black; text-align: center"
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|3
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| <html>
<a href="http://partsregistry.org/Part:BBa_K233005"> Part: BBa_K233005</a>
<a href="http://partsregistry.org/Part:BBa_K233005"> Part: BBa_K233005</a>
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</html> Still in the design stage. We will try to create this DNA part in our lab.
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</html>
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|AND gate 2
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6) <html>
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| phage protein ogr regulated AND GATE
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|-
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|- style="background:#66ffcc; color:black; text-align: center"
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|4
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|<html>
<a href="http://partsregistry.org/Part:BBa_K233006"> Part: BBa_K233006</a>
<a href="http://partsregistry.org/Part:BBa_K233006"> Part: BBa_K233006</a>
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</html> Still in the design stage. We will try to create this DNA part in our lab.
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</html>  
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|AND Gate 3
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7) <html>
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|Phage T7 polymerase regulated And Gate
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<a href="http://partsregistry.org/Part:BBa_K233008"> Part: BBa_K233008</a>
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|-
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</html> We have successfully obtained and shipped this part. It is a GFP secretion device.
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|- style="background:#66ffcc; color:black; text-align: center"
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|5
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8)<html>
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|<html>
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<a href="http://partsregistry.org/Part:BBa_BBa_K233306"> Part: BBa_K233306</a>
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<a href="http://partsregistry.org/Part:BBa_K233318"> Part: BBa_K233318</a>
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</html>We have successfully obtained and shipped this part. YcdB is one of our two Export Tags.
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</html>  
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|Gfp exporter 1
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9)<html>
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|A tester for Snowdrift 1
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<a href="http://partsregistry.org/Part:BBa_K233307"> Part: BBa_K233307</a>
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|-
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</html>We have successfully obtained and shipped this part. TorA is the second of our Export Tags.
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|- style="background:#66ffcc; color:black; text-align: center"
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|6
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10)<html>
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|<html>
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<a href="http://partsregistry.org/Part:BBa_K233309"> Part: BBa_K233309</a>
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<a href="http://partsregistry.org/Part:BBa_K233319"> Part: BBa_K233319</a>
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</html>We have successfully obtained and shipped this part. It is a GFP secretion device.
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</html>  
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|Gfp exporter 2
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11)<html>
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|A tester for Snowdrift 2
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<a href="http://partsregistry.org/Part:BBa_K233310"> Part: BBa_K233310</a>
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|-
-
</html>We have successfully obtained and shipped this part. It is a constitutive green fluorescent protein device
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|- style="background:#66ffcc; color:black; text-align: center"
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|7
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12)<html>
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|<html>
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<a href="http://partsregistry.org/Part:BBa_K233311"> Part: BBa_K233311</a>
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<a href="http://partsregistry.org/Part:BBa_K233320"> Part: BBa_K233320</a>
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</html>We have successfully obtained and shipped this part. It is a Protein(GFP) secretion device.
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</html>  
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|Turing Machine- Unary Adder
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13)<html>
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|The simplified construct for the Turing machine
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<a href="http://partsregistry.org/Part:BBa_K233313"> Part: BBa_K233313</a>
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|-
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</html>We have successfully obtained and shipped this part. It is an AHL induced AHL producer
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|- style="background:#66ffcc; color:black; text-align: center"
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|8
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14)<html>
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|<html>
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<a href="http://partsregistry.org/Part:BBa_K233314"> Part: BBa_K233314</a>
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<a href="http://partsregistry.org/Part:BBa_K233321"> Part: BBa_K233321</a>
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</html>We have successfully obtained and shipped this part. It is a constitutive LuxR production device
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</html>  
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|Turing Machine- Unary Adder 2
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15)<html>
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|The simplified construct for the Turing Machine 2
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<a href="http://partsregistry.org/Part:BBa_K233315"> Part: BBa_K233315</a>
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|-
-
</html>We have successfully obtained and shipped this part. It is a constitutive LuxR generating device
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|- style="background:#66ffcc; color:black; text-align: center"
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|9
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16)<html>
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|<html>
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<a href="http://partsregistry.org/Part:BBa_K233316"> Part: BBa_K233316</a>
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<a href="http://partsregistry.org/Part:BBa_K233322"> Part: BBa_K233322</a>
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</html>We have successfully obtained and shipped this part. It is an intermediate part for our AND gate, helping us characterise the AND gates.
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</html>  
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|Turing machine cassette 2
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17)<html>
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|AHL and Lactose Modulated Cell Lysis
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<a href="http://partsregistry.org/Part:BBa_K233317"> Part: BBa_K233317</a>
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|-
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</html>We have successfully obtained and shipped this part. It is an RFP measurement device
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|- style="background:#66ffcc; color:black; text-align: center"
 +
|10
 +
|<html>
 +
<a href="http://partsregistry.org/Part:BBa_K233323"> Part: BBa_K233323</a>
 +
</html>  
 +
|Turing machine cassette 2
 +
|AHL and Lactose modulated Gfp production
 +
|-
 +
|- style="background:#66ffcc; color:black; text-align: center"
 +
|11
 +
|<html>
 +
<a href="http://partsregistry.org/Part:BBa_K233324"> Part: BBa_K233324</a>
 +
</html>  
 +
|pIDTSmart-KAN Vector
 +
|The Backbone of our favorite parts
 +
|-style="background:#66ffcc; color:black; text-align: center"  
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|12
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|<html>
 +
<a href="http://partsregistry.org/Part:BBa_K233325"> Part: BBa_K233325</a>
 +
</html>  
 +
|The Complete Turing Machine Construct
 +
|The Complete Turing Machine Construct
 +
|-style="background:#66ffcc; color:black; text-align: center"  
 +
|}

Latest revision as of 01:29, 22 October 2009






Parts shipped to the registry

"width: 1400 px"
Sr No. BioBrick Name Part Name TYPE Description Backbone LENGTH (bp) SEQUENCED
1 BBa_K233306 YcdB Signalling YcdB - This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT) BBa_K233324 123 Yes
2 BBa_K233004 lac operator regulatory Lac operator. BBa_K233324 30 Yes
3 BBa_K233307 TorA Signalling TorA- This part is a export tag that utilizes the Twin Arginine Transport pathway(TAT) BBa_K233324 139 Yes
4 BBa_K233008 TorA-GFP Signalling This construct is an intermediate in the production of an exportable GFP. pSB1AK3 865 -
5 BBa_K233309 YcdB-GFP Signalling This is an intermediate which can be used for constructing a module which can be used to produce an exportable GFP pSB1AK3 849 -
6 BBa_K233310 constitutive GFP device Reporter Promoter-RBS-GFP- constitutive green fluorescent protein device pSB1AK3 800 -
7 BBa_K233311 Protein(GFP) secretion device Device This device codes for an exportable GFP pSB1AK3 945 -
8 BBa_K233312 GFP secretion device using Ycdb Device This device codes for an exportable GFP pSB1AK3 929 -
9 BBa_K233313 ahl induced ahl producer Composite construct designed to produce AHL (a quorum sensing molecule) in response to environmental AHL pSB1AK3 725 -
10 BBa_K233314 constitutive luxR production device Generator This part allows constitutive production of the LuxR protein which binds to AHL and the LuxR-AHL complex activates the pLuxR promoter. pSB1A2 881 -
11 BBa_K233315 constitutive LuxR generating device Generator This part allows constitutive production of the LuxR protein pSB1A2 824 -
12 BBa_K233316 Constitutive promoter lac operator Device This part can be used to calibrate the working of the lac operator pSB1A2 73 -
13 BBa_K233317 rfp measurement device measurement This part can be used to calibrate the working of BBa_J13002 pSB1A2 912 -
14 BBa_K233003 AND Gate 1 Regulatory This is an AND Gate, responsive to two external inputs, Lactose and AHL. pSB1C3 94 -


Parts designed by us


Sr No. BioBrick Name Part Name Description
1 Part: BBa_K233002 Snowdrift 2 , using YcdB. A module to secrete B-galactosidase using the TAT-export Pathway
2 Part: BBa_K233001 Snowdrift 1 , using TorA A module to secrete B-galactosidase using the TAT-export Pathway
3 Part: BBa_K233005 AND gate 2 phage protein ogr regulated AND GATE
4 Part: BBa_K233006 AND Gate 3 Phage T7 polymerase regulated And Gate
5 Part: BBa_K233318 Gfp exporter 1 A tester for Snowdrift 1
6 Part: BBa_K233319 Gfp exporter 2 A tester for Snowdrift 2
7 Part: BBa_K233320 Turing Machine- Unary Adder The simplified construct for the Turing machine
8 Part: BBa_K233321 Turing Machine- Unary Adder 2 The simplified construct for the Turing Machine 2
9 Part: BBa_K233322 Turing machine cassette 2 AHL and Lactose Modulated Cell Lysis
10 Part: BBa_K233323 Turing machine cassette 2 AHL and Lactose modulated Gfp production
11 Part: BBa_K233324 pIDTSmart-KAN Vector The Backbone of our favorite parts
12 Part: BBa_K233325 The Complete Turing Machine Construct The Complete Turing Machine Construct
Retrieved from "http://2009.igem.org/Team:IBB_Pune/Parts"