Team:Imperial College London/Genetic Circuit

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=Genetic Circuits=
=Genetic Circuits=
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Here we go through the genetic circuit of <b><i>The E.ncapsulator</i></b> system. The design of the circuit has been  kept very modular to facilitate reusability.<br>
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Here, the full genetic circuit of <b><i>The E.ncapsulator</i></b> system can be seen. In order to benefit future teams wishing to reuse the features found in our project, a modular approach was taken at every level of the system design. In addition, integration modules have been designed and incorporated, providing a means to link the respective modules, without the use of a timer as one would expect.
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We will go through the genetic design of the system, module by module. If you are unfamiliar with genetic circuit diagrams, scroll down to the bottom of the page to check out our
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<html>
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<a href="#" onclick="return hs.htmlExpand(this, {
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width: 400, creditsPosition: 'bottom left',
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headingText: 'Key to our genetic circuit diagrams', wrapperClassName: 'titlebar' } )">Inline HTML</a>
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<img src="https://static.igem.org/mediawiki/2009/7/78/II09_GC_Key.png">
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</html>to familiarise yourself with each circuit symbol and what it means. Otherwise scroll down to view our [[Team:Imperial_College_London/Genetic_Circuit#Modular_Genetic_Circuits|modular genetic circuits]].
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This page will start by going through the genetic circuit design of the system, module by module.
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<a href="https://static.igem.org/mediawiki/2009/7/78/II09_GC_Key.png" class="highslide" onclick="return hs.expand(this)">
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<img src="https://static.igem.org/mediawiki/2009/7/78/II09_GC_Key.png" alt="" title="Key to Genetic Circuit Diagrams, see below for more information about each circuit symbol" width="20%"/>
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==Modular Genetic Circuits==
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<center><b><i>Click on each genetic circuit to learn more about them individually. </i></b></center>
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Key to genetic circuit diagrams
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<br><br>
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===Modular Genetic Circuits===
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<center><b><i>Click on each genetic circuit to learn more about them. </i></b></center>
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<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M1/Genetic"><img style="vertical-align:bottom;" width=700px align="left" src="https://static.igem.org/mediawiki/2009/d/d3/M1gci.jpg"></a>
<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/M1/Genetic"><img style="vertical-align:bottom;" width=700px align="left" src="https://static.igem.org/mediawiki/2009/d/d3/M1gci.jpg"></a>
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===Construction of Circuits===
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{{Imperial/09/Division}}
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The genetic circuits were created in a logical step-by-step approach following the design of a unique cloning strategy. Smaller constructs were first created, and these built up to form the whole circuit. In order to allow for testing and part characterisation at various stages of the project, a number of intermediate testing constructs were also designed and built.
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===Key to Genetic Circuit Diagrams===
 
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This key explains some of the symbols used for the genetic circuit diagrams.<br><br>
 
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[[Image:II09_GC_Key.png|425px|center]]
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<html><a href="https://2009.igem.org/Team:Imperial_College_London/Wetlab/Cloning_Strategy"><img style="vertical-align:bottom;" width=50px align="left" src="http://i691.photobucket.com/albums/vv271/dk806/II09Learnmore.png"></a></html><b>&nbsp; About our step-by-step cloning strategy</b>
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<br><br>
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A <b>promoter</b> is a genetic element in DNA that regulates the transcription of a downstream gene. Promoters can be induced or repressed by external factors and the expression of the downstream gene is consequently under control of the same external factors. We use promoters as the control mechanisms under which the expression of our genes is controlled. <br>
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An <b>RBS</b> is a ribosome binding site. This is a region of DNA to which the ribosome binds in the initiation of protein translation. An RBS is required adjacent to each coding sequence to in order to have expression.<br>
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A <b>Protein Coding Sequence</b> is commonly referred to as a gene. This is a section of DNA of which the nucleotide sequence is translated by ribosomes into the amino acid sequence of proteins. <br>
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<b>Terminators</b> are required at the end of each operon (genes under control of one promoter) in order to signal to the RNA polymerase that the operon has finished, and to release from the DNA strand during DNA transcription. We have used double terminators as standard to improve the efficiency.<br><br><br>
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{{Imperial/09/Division}}
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== BioBrick Submissions ==
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This year, Imperial College London have added 29 new parts to the BioBrick registry. In addition we sought to characterise some of these parts, including the thermosensitive BBa_K098995 construct which has now been made functional by us. Detailed information about the characterisation of some of our parts can be found in the Major Results page.
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About the characterisation of the BBa_K098995 construct.
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===Here are the parts Imperial College have submitted this year:===
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<html><iframe src="http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=Imperial%20College%20London" width="100%" height="600px"></iframe></html>
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===Project Tour===
===Project Tour===
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<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control"><img width=150px src="http://i691.photobucket.com/albums/vv271/dk806/TemporalControlL.jpg"></a>
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<html><center><a href="https://2009.igem.org/Team:Imperial_College_London/Temporal_Control"><img width=150px src="https://static.igem.org/mediawiki/2009/f/f0/II09_Gen_ArrowLeft.png"></a></center></html>
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<a href="https://2009.igem.org/Team:Imperial_College_London/Summary"><img width=150px src="http://i691.photobucket.com/albums/vv271/dk806/SummaryAchR.jpg"></a>
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{{Imperial/09/TemplateBottom}}
{{Imperial/09/TemplateBottom}}

Latest revision as of 21:13, 20 October 2009

Contents

Genetic Circuits

Here, the full genetic circuit of The E.ncapsulator system can be seen. In order to benefit future teams wishing to reuse the features found in our project, a modular approach was taken at every level of the system design. In addition, integration modules have been designed and incorporated, providing a means to link the respective modules, without the use of a timer as one would expect.

This page will start by going through the genetic circuit design of the system, module by module.


Modular Genetic Circuits

Click on each genetic circuit to learn more about them individually.






Construction of Circuits

The genetic circuits were created in a logical step-by-step approach following the design of a unique cloning strategy. Smaller constructs were first created, and these built up to form the whole circuit. In order to allow for testing and part characterisation at various stages of the project, a number of intermediate testing constructs were also designed and built.


  About our step-by-step cloning strategy

BioBrick Submissions

This year, Imperial College London have added 29 new parts to the BioBrick registry. In addition we sought to characterise some of these parts, including the thermosensitive BBa_K098995 construct which has now been made functional by us. Detailed information about the characterisation of some of our parts can be found in the Major Results page.

About the characterisation of the BBa_K098995 construct.

Here are the parts Imperial College have submitted this year:




Project Tour

Mr. Gene   Geneart   Clontech   Giant Microbes
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