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Team:Kyoto/CiC/Notebook/0907-0911
From 2009.igem.org
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!NO.!!Sample name!!conc./(ng/ul) | !NO.!!Sample name!!conc./(ng/ul) | ||
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| - | |1|| | + | |1||HIV-TAT-(LALAAAA)3(EcoR1,Spe1)||36.2 |
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Revision as of 09:51, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator
Wednesday, 16 September
To Do
- PCR
- HIV-TAT-(LALAAAA)3
- Restriction Enzyme Digestion
- HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
- B0015 (EcoR1 + Spe1)
- Electrophoresis
Results
Fryday, 11 September
To Do
- Ligation
- Transformation
Results
ligation
| NO. | Sample name | conc./(ng/ul) |
|---|---|---|
| 1 | HIV-TAT-(LALAAAA)3(EcoR1,Spe1) | 36.2 |
Transformation
No colony was observed (090917 10:00)
