Team:Kyoto/CiC/Notebook/0907-0911

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Revision as of 09:53, 21 October 2009

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator

Wednesday, 16 September

To Do

  • PCR
    • HIV-TAT-(LALAAAA)3
  • Restriction Enzyme Digestion
    • HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
    • B0015 (EcoR1 + Spe1)
  • Electrophoresis

Results

Fryday, 11 September

To Do

  • Ligation
  • Transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
1HIV-TAT-(LALAAAA)3(EcoR1,Spe1)sample136.2 1HIV-TAT-(LALAAAA)3(EcoR1,Spe1)sample136.2
Transformation

No colony was observed (090917 10:00)　

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