Team:Kyoto/CiC/Notebook/0907-0911

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===Wednesday, 16 September===
===Wednesday, 16 September===
====To Do====
====To Do====
-
*PCR
+
*HIV-TAT-(LALAAAA)3
-
**HIV-TAT-(LALAAAA)3
+
**PCR
-
*Restriction Enzyme Digestion
+
**Restriction Enzyme Digestion(EcoR1 + Spe1)
-
**HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
+
*B0015
-
**B0015 (EcoR1 + Spe1)
+
**Restriction Enzyme Digestion(EcoR1 + Xba1)
-
*Electrophoresis
+
====Results====
====Results====
-
 
+
*Band of expected size were observed.
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
===Fryday, 11 September===
===Fryday, 11 September===
====To Do====
====To Do====
-
*Ligation
+
*HIV-TAT-(LALAAAA)3
-
*Transformation
+
**Ligation
 +
**Transformation
====Results====
====Results====
=====ligation=====
=====ligation=====
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!NO.!!Sample name!!conc./(ng/ul)
!NO.!!Sample name!!conc./(ng/ul)
|-
|-
-
|1||HIV-TAT-(LALAAAA)3 sample1(EcoR1,Spe1)||36.2
+
|1||HIV-TAT sample1(EcoR1,Spe1)||36.2
|-
|-
-
|2||HIV-TAT-(LALAAAA)3 sample2(EcoR1,Spe1)||21.7
+
|2||HIV-TAT sample2(EcoR1,Spe1)||21.7
|-
|-
-
|3||HIV-TAT-(LALAAAA)3 sample3(EcoR1,Spe1)||47.9
+
|3||HIV-TAT sample3(EcoR1,Spe1)||47.9
|-
|-
|4||B0015(EcoR1,Xba1)||2.48
|4||B0015(EcoR1,Xba1)||2.48
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=====Transformation=====
=====Transformation=====
-
No colony was observed (090917 10:00)　
+
Colony was observed (090912)　
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}

Latest revision as of 16:15, 21 October 2009

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator

Wednesday, 16 September

To Do

  • HIV-TAT-(LALAAAA)3
    • PCR
    • Restriction Enzyme Digestion(EcoR1 + Spe1)
  • B0015
    • Restriction Enzyme Digestion(EcoR1 + Xba1)

Results

  • Band of expected size were observed.

Fryday, 11 September

To Do

  • HIV-TAT-(LALAAAA)3
    • Ligation
    • Transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
1HIV-TAT sample1(EcoR1,Spe1)36.2
2HIV-TAT sample2(EcoR1,Spe1)21.7
3HIV-TAT sample3(EcoR1,Spe1)47.9
4B0015(EcoR1,Xba1)2.48
Transformation

Colony was observed (090912)　

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