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Team:Kyoto/CiC/Notebook/0907-0911
From 2009.igem.org
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Revision as of 10:01, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator
Wednesday, 16 September
To Do
- PCR
- HIV-TAT-(LALAAAA)3
- Restriction Enzyme Digestion
- HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
- B0015 (EcoR1 + Spe1)
- Electrophoresis
Results
Fryday, 11 September
To Do
- Ligation
- Transformation
Results
ligation
| NO. | Sample name | conc./(ng/ul) |
|---|---|---|
| 1 | HIV-TAT-(LALAAAA)3 sample1(EcoR1,Spe1) | 36.2 |
| 2 | HIV-TAT-(LALAAAA)3 sample2(EcoR1,Spe1) | 21.7 |
| 3 | HIV-TAT-(LALAAAA)3 sample3(EcoR1,Spe1) | 47.9 |
| 4 | B0015(EcoR1,Xba1) | 2.48 |
Transformation
Colony was observed (090912)
