Team:Kyoto/CiC/Notebook/0907-0911

From 2009.igem.org

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(→Thursday, 17 September)
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*Ligation
*Ligation
*Transformation
*Transformation
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====Results====
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=====ligation=====
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{| class="table"
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|+conc.
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!NO.!!Sample name!!conc./(ng/ul)
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|-
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|1||11(stu1)||103.2
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|}
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=====Transformation=====
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No colony was observed (090917 10:00)　
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{{:Team:Kyoto/Pad_between_dates}}

Revision as of 09:50, 21 October 2009

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  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

097-0911 : Plasmid Construction of HIV-TAT-(LALAAAA)3-generator

Wednesday, 16 September

To Do

  • PCR
    • HIV-TAT-(LALAAAA)3
  • Restriction Enzyme Digestion
    • HIV-TAT-(LALAAAA)3 (EcoR1 + Spe1)
    • B0015 (EcoR1 + Spe1)
  • Electrophoresis

Results

Fryday, 11 September

To Do

  • Ligation
  • Transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00)　

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/0907-0911"