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Team:Kyoto/CiC/Notebook/0914-0918
From 2009.igem.org
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===Wednesday, 16 September=== | ===Wednesday, 16 September=== | ||
====To Do==== | ====To Do==== | ||
| - | *HIV-TAT-(LALAAAA)3+B0015 | + | *HIV-TAT-(LALAAAA)3+B0015(terminator) |
**miniprep | **miniprep | ||
**Restriction Enzyme Digestion(Xba1,Pst1) | **Restriction Enzyme Digestion(Xba1,Pst1) | ||
Revision as of 10:13, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
0914-0918 : what to do. what to do. what to do. what to do.
Monday, 14 September
To Do
- わからん
Results
Tuesday, 15 September
To Do
- 知らん
Wednesday, 16 September
To Do
- HIV-TAT-(LALAAAA)3+B0015(terminator)
- miniprep
- Restriction Enzyme Digestion(Xba1,Pst1)
- I712074(T7promoter)
- Restriction Enzyme Digestion(Spe1,Pst1)
Results
ligation
| NO. | Sample name | conc./(ng/ul) |
|---|---|---|
| 1 | 11(stu1) | 103.2 |
Transformation
No colony was observed (090917 10:00)
Thursday, 17 September
To Do
- Make repetitive sequence (MPR)
- Transformation(retry)
