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Team:Kyoto/CiC/Notebook/0914-0918
From 2009.igem.org
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| - | + | *ligationの濃度データがない | |
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=====Transformation===== | =====Transformation===== | ||
| - | + | Colony was observed (090917) | |
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
Revision as of 10:19, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
0914-0918 : what to do. what to do. what to do. what to do.
Monday, 14 September
To Do
- わからん
Results
Tuesday, 15 September
To Do
- 知らん
Wednesday, 16 September
To Do
- HIV-TAT-(LALAAAA)3+B0015(terminator)
- miniprep
- Restriction Enzyme Digestion(Xba1,Pst1)
- ligation
- I712074(T7promoter)
- Restriction Enzyme Digestion(Spe1,Pst1)
- ligation
Results
- ligationの濃度データがない
Transformation
Colony was observed (090917)
Thursday, 17 September
To Do
- Make repetitive sequence (MPR)
- Transformation(retry)
