Team:Kyoto/CiC/Notebook/0914-0918

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==0914-0918 : what to do. what to do. what to do. what to do. ==
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==0914-0918 : constructing HIV-TAT-(LALAAAA)3-generator  ==
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===Monday, 14 September===
===Monday, 14 September===
====To Do====
====To Do====
-
*わからん
+
*No experiments were undertaken
-
====Results====
+
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===Tuesday, 15 September===
===Tuesday, 15 September===
====To Do====
====To Do====
-
'''Further to 14 Sept.'''
+
*HIV-TAT-(LALAAAA)3-generator
-
 
+
-
*p-2-(1)(4)(7)(8)(10)
+
-
**Miniprep
+
-
**Restriction Enzyme Digestion
+
-
*p-2-(1)(4)  E.X
+
-
**PCR purification kit
+
-
*p-2-(1)(7)(8)(10)  E.S
+
-
**Gel extraction
+
-
*4-(1)(2)(3)
+
**Colony PCR
**Colony PCR
-
**Insert check
+
====Results====
-
 
+
Bands of expected size were observed.
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 +
===Wednesday, 16 September===
===Wednesday, 16 September===
====To Do====
====To Do====
 +
*HIV-TAT-(LALAAAA)3+B0015(terminator)
 +
**miniprep
 +
**Restriction Enzyme Digestion(Xba1,Pst1)
 +
**ligation
 +
*I712074(T7promoter)
 +
**Restriction Enzyme Digestion(Spe1,Pst1)
**ligation
**ligation
-
**transformation
 
====Results====
====Results====
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=====ligation=====
 
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{| class="table"
 
-
|+conc.
 
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!NO.!!Sample name!!conc./(ng/ul)
 
-
|-
 
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|1||11(stu1)||103.2
 
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|}
 
-
 
=====Transformation=====
=====Transformation=====
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No colony was observed (090917 10:00)　
+
Colony was observed (090917)　
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===Thursday, 17 September===
===Thursday, 17 September===
====To Do====
====To Do====
-
*Make repetitive sequence (MPR)
+
*HIV-TAT-(LALAAAA)3-generator
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*Transformation(retry)
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**colony PCR
 +
====Results====
 +
*Binds of expected size were observed.
 +
 
 +
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 +
 
 +
===Friday, 18 September===
 +
====To Do====
 +
*No experiments were undertaken
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Latest revision as of 19:32, 21 October 2009

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  2. CiC
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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : constructing HIV-TAT-(LALAAAA)3-generator

Monday, 14 September

To Do

  • No experiments were undertaken

Tuesday, 15 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

Bands of expected size were observed.

Wednesday, 16 September

To Do

  • HIV-TAT-(LALAAAA)3+B0015(terminator)
    • miniprep
    • Restriction Enzyme Digestion(Xba1,Pst1)
    • ligation
  • I712074(T7promoter)
    • Restriction Enzyme Digestion(Spe1,Pst1)
    • ligation

Results

Transformation

Colony was observed (090917)　

Thursday, 17 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • colony PCR

Results

  • Binds of expected size were observed.

Friday, 18 September

To Do

  • No experiments were undertaken

Retrieved from "http://2009.igem.org/Team:Kyoto/CiC/Notebook/0914-0918"