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Team:Kyoto/CiC/Notebook/0914-0918
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| - | ==0914-0918 : | + | ==0914-0918 : constructing HIV-TAT-(LALAAAA)3-generator == |
{{:Team:Kyoto/Pad_start_dates}} | {{:Team:Kyoto/Pad_start_dates}} | ||
===Monday, 14 September=== | ===Monday, 14 September=== | ||
====To Do==== | ====To Do==== | ||
| - | * | + | *No experiments were undertaken |
| - | + | ||
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
===Tuesday, 15 September=== | ===Tuesday, 15 September=== | ||
====To Do==== | ====To Do==== | ||
| - | * | + | *HIV-TAT-(LALAAAA)3-generator |
| + | **Colony PCR | ||
| + | ====Results==== | ||
| + | Bands of expected size were observed. | ||
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
Latest revision as of 19:32, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
0914-0918 : constructing HIV-TAT-(LALAAAA)3-generator
Monday, 14 September
To Do
- No experiments were undertaken
Tuesday, 15 September
To Do
- HIV-TAT-(LALAAAA)3-generator
- Colony PCR
Results
Bands of expected size were observed.
Wednesday, 16 September
To Do
- HIV-TAT-(LALAAAA)3+B0015(terminator)
- miniprep
- Restriction Enzyme Digestion(Xba1,Pst1)
- ligation
- I712074(T7promoter)
- Restriction Enzyme Digestion(Spe1,Pst1)
- ligation
Results
Transformation
Colony was observed (090917)
Thursday, 17 September
To Do
- HIV-TAT-(LALAAAA)3-generator
- colony PCR
Results
- Binds of expected size were observed.
Friday, 18 September
To Do
- No experiments were undertaken
