Team:Kyoto/CiC/Notebook/0921-0925

From 2009.igem.org

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-
==0914-0918 : what to do. what to do. what to do. what to do. ==
+
==0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)  ==
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===Monday, 21 September===
===Monday, 21 September===
====To Do====
====To Do====
-
*No experiments were undertaken
+
*HIV-TAT-(LALAAAA)3-generator
 +
**Colony PCR
 +
====Results====
 +
*Binds of expected size were observed.
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*HIV-TAT-(LALAAAA)3-generator
*HIV-TAT-(LALAAAA)3-generator
**miniprep
**miniprep
-
*TIM23complex signal::EGFP generator
+
*sig-EGFP generator(HeLa)
-
**PEGFP-N3 VECTOR (EGFP generator)
+
**Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
-
====Results====
+
**hosphorylation signal sequence primer
-
*消えた
+
**annering signal sequence primer
 +
 
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===Wednesday, 23 September===
===Wednesday, 23 September===
====To Do====
====To Do====
 +
*sig-EGFP generator
**ligation
**ligation
**transformation
**transformation
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{| class="table"
{| class="table"
|+conc.
|+conc.
-
!NO.!!Sample name!!conc./(ng/ul)
+
!NO.!!Sample name!!conc./(ng/ul)!!conc.(ul)
 +
|-
 +
|1||pEGFP-N3(EcoR1,Xho1)||224.3||1
|-
|-
-
|1||11(stu1)||103.2
+
|2||signal sequence||0.3||11
|}
|}
=====Transformation=====
=====Transformation=====
-
No colony was observed (090917 10:00) 
+
No colony was observed. (090917 10:00) 
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Latest revision as of 02:06, 22 October 2009

  1. Home
  2. CiC
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : constructing sig-GFP generator,sig-EGFP generator(HeLa)


Monday, 21 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

  • Binds of expected size were observed.



Tuesday, 22 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • miniprep
  • sig-EGFP generator(HeLa)
    • Restriction Enzyme PEGFP-N3 VECTOR (EGFP generator)
    • hosphorylation signal sequence primer
    • annering signal sequence primer



Wednesday, 23 September

To Do

  • sig-EGFP generator
    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)conc.(ul)
1pEGFP-N3(EcoR1,Xho1)224.31
2signal sequence0.311
Transformation

No colony was observed. (090917 10:00) 



Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)