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Team:Kyoto/CiC/Notebook/1005-1009
From 2009.igem.org
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===Saturday, 11 October=== | ===Saturday, 11 October=== | ||
====To Do==== | ====To Do==== | ||
| + | *Yeast Mitochondria isolation | ||
====Results==== | ====Results==== | ||
| + | *It is too difficult for us to isolate mitochondoria. | ||
{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
Revision as of 15:55, 21 October 2009
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
1005-1009 : what to do. what to do. what to do. what to do.
Monday, 05 October
To Do
No experiments were undertaken
Tuesday, 06 October
To Do
No experiments were undertaken
Wednesday, 07 October
To Do
- sig-GFP generator
- Restriction Enzyme
- ligation
- transformation
- sig-EGFP generator
- phosphorylation signal sequence primer
- anneling signal sequence primer
- ligation
- transformation
Results
Transformation
Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)
Thursday, 08 October
To Do
- sig-GFP generator
- Restriction Enzyme
- ligation
- transformation
- sig-EGFP generator
- phosphorylation signal sequence primer
- anneling signal sequence primer
- ligation
- transformation
Results
Transformation
Sufficient,but not excessive number of colonies were observed,so we decided to move on to next step.(091010)
Friday, 09 October
To Do
- No experiments were undertaken
Saturday, 10 October
To Do
- sig-GFP
- miniprep
- Restriction Enzyme
- sig-EGFP(HeLa)
- miniprep
- Restriction Enzyme
Results
Plasmid construction is completed!!
Saturday, 11 October
To Do
- Yeast Mitochondria isolation
Results
- It is too difficult for us to isolate mitochondoria.
Sunday, 12 October
To Do
Results
