Team:Kyoto/CiC/Notebook/1005-1009

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

1005-1009 : what to do. what to do. what to do. what to do.


Monday, 05 October

To Do

No experiments were undertaken



Tuesday, 06 October

To Do

No experiments were undertaken



Wednesday, 07 October

To Do

  • sig-GFP generator
    • Restriction Enzyme
    • ligation
    • transformation
  • sig-EGFP generator
    • phosphorylation signal sequence primer
    • anneling signal sequence primer
    • ligation
    • transformation

Results

Transformation

Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)



Thursday, 08 October

To Do

  • sig-GFP generator
    • Restriction Enzyme
    • ligation
    • transformation
  • sig-EGFP generator
    • phosphorylation signal sequence primer
    • anneling signal sequence primer
    • ligation
    • transformation

Results

Transformation

Too many colonies were observed that we could find no fignificant differences between the treated culuture the control. (091008)



Friday, 09 October

To Do

  • No experiments were undertaken



Saturday, 10 October

To Do

Results



Saturday, 11 October

To Do

Results



Sunday, 12 October

To Do

Results