Team:Kyoto/CiC/Notebook/1019-1023

From 2009.igem.org

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(To Do)
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====To Do====
====To Do====
* subgoal A --- Confirmation of function of TAT-liposome
* subgoal A --- Confirmation of function of TAT-liposome
 +
**observation by microscope
* Subgoal B --- Liposomes with Mitochondrial Translocases
* Subgoal B --- Liposomes with Mitochondrial Translocases
-
* Subgoal C --- sig-GFP expression in HeLa
+
**Mitochondoria isolation
 +
**expressing sig-GFP in vitro with mitochondoria
 +
* Subgoal C --- sig-EGFP expression in HeLa
 +
**transfomation
====Results====
====Results====

Revision as of 20:00, 21 October 2009

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

1019-1025 :Obserbation


Monday, 19 October

To Do

  • subgoal A --- Confirmation of function of TAT-liposome
    • observation by microscope
  • Subgoal B --- Liposomes with Mitochondrial Translocases
    • Mitochondoria isolation
    • expressing sig-GFP in vitro with mitochondoria
  • Subgoal C --- sig-EGFP expression in HeLa
    • transfomation

Results



Tuesday, 20 October

To Do

Results



Wednesday, 21 October

To Do

Results



Thursday, 22 October

To Do

Results



Friday, 23 October

To Do

Results



Saturday, 24 October

To Do

Results



Saturday, 25 October

To Do

Results



Sunday, 26 October

To Do

Results