Team:Kyoto/CiC/Notebook/week1

From 2009.igem.org

(Difference between revisions)
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==Week 1 : what to do. what to do. what to do. what to do. ==
==Week 1 : what to do. what to do. what to do. what to do. ==
{{:Team:Kyoto/Pad_start_dates}}
{{:Team:Kyoto/Pad_start_dates}}
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===Monday, June 9===
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===Monday, September 10===
====To Do on 24 July====
====To Do on 24 July====
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*Miniprep (3-O-2, 3-A-4)
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*PCR (HIV TAT LALAA peptide,11R LALLA peptide)
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*Restriction Enzyme Digestion
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*Electrophoresis (HIV TAT LALAA peptide,11R LALLA peptide)
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*Electrophoresis
+
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====Results====
====Results====
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=====Miniprep=====
 
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*3-O-2, 3-A-4 was minipreped.
 
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=====Digestion=====
 
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*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
 
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{| border="1" style="text-align:center;width:50%"
 
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|+Sample list
 
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!NO.!!Sample Name!!Enzyme
 
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|-align="center"
 
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|1||3-O-2||Eco R1, PST1
 
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|-align="center"
 
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|2||3-A-4||Eco R1, PST1
 
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|-align="center"
 
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|3||3-O-2||Eco R1
 
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|-align="center"
 
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|4||3-A-4||Eco R1
 
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|}
 
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=====Electrophoresis=====
=====Electrophoresis=====
[[Image:Kyoto_e_090724_1.png|250px]]
[[Image:Kyoto_e_090724_1.png|250px]]
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{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
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===Tuseday, June 10===
===Tuseday, June 10===
====To Do on 24 July====
====To Do on 24 July====

Revision as of 18:30, 20 October 2009

  1. Home
  2. Cells in Cells
  3. Notebook

0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

Week 1 : what to do. what to do. what to do. what to do.

Monday, September 10

To Do on 24 July

  • PCR (HIV TAT LALAA peptide,11R LALLA peptide)
  • Electrophoresis (HIV TAT LALAA peptide,11R LALLA peptide)

Results

Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266

Tuseday, June 10

To Do on 24 July

  • Miniprep (3-O-2, 3-A-4)
  • Restriction Enzyme Digestion
  • Electrophoresis

Results

Miniprep
  • 3-O-2, 3-A-4 was minipreped.
Digestion
  • Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
13-O-2Eco R1, PST1
23-A-4Eco R1, PST1
33-O-2Eco R1
43-A-4Eco R1
Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266

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