Team:Kyoto/CiC/Notebook/week1

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(→Week 1 : what to do. what to do. what to do. what to do.)
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====To Do on 24 July====
====To Do on 24 July====
*PCR (HIV TAT LALAA peptide,11R LALLA peptide)
*PCR (HIV TAT LALAA peptide,11R LALLA peptide)
-
*Electrophoresis (HIV TAT LALAA peptide,11R LALLA peptide)
+
*Electrophoresis
 +
*Restriction Enzyme Digestion
====Results====
====Results====
=====Electrophoresis=====
=====Electrophoresis=====
Line 49: Line 50:
|Plasmid length / bp||3266||3266
|Plasmid length / bp||3266||3266
|}
|}
 +
 +
=====Digestion=====
 +
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
 +
 +
{| border="1" style="text-align:center;width:50%"
 +
|+Sample list
 +
!NO.!!Sample Name!!Enzyme
 +
|-align="center"
 +
|1||3-O-2||Eco R1, PST1
 +
|-align="center"
 +
|2||3-A-4||Eco R1, PST1
 +
|-align="center"
 +
|3||3-O-2||Eco R1
 +
|-align="center"
 +
|4||3-A-4||Eco R1
 +
|}
 +
 +
{{:Team:Kyoto/Pad_between_dates}}
{{:Team:Kyoto/Pad_between_dates}}
-
===Tuseday, June 10===
+
 
 +
 
 +
===Tuseday, September 11===
====To Do on 24 July====
====To Do on 24 July====
-
*Miniprep (3-O-2, 3-A-4)
+
*Ligation(HIV TAT LALAA peptide,11R LALLA peptide)  
-
*Restriction Enzyme Digestion
+
*Transformation
-
*Electrophoresis
+
====Results====
====Results====
-
=====Miniprep=====
+
=====Ligation=====
-
*3-O-2, 3-A-4 was minipreped.
+
-
 
+
-
=====Digestion=====
+
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.

Latest revision as of 18:43, 20 October 2009

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide

Monday, September 10

To Do on 24 July

  • PCR (HIV TAT LALAA peptide,11R LALLA peptide)
  • Electrophoresis
  • Restriction Enzyme Digestion

Results

Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266
Digestion
  • Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
13-O-2Eco R1, PST1
23-A-4Eco R1, PST1
33-O-2Eco R1
43-A-4Eco R1



Tuseday, September 11

To Do on 24 July

  • Ligation(HIV TAT LALAA peptide,11R LALLA peptide)
  • Transformation

Results

Ligation
  • Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
Sample list
NO.Sample NameEnzyme
13-O-2Eco R1, PST1
23-A-4Eco R1, PST1
33-O-2Eco R1
43-A-4Eco R1
Electrophoresis

Kyoto e 090724 1.png

  • Very clear bands were found. (See the gel image in right!)
  • The bands are listed below.
Parts and Plasmid length
3-O-23-A-4
Parts length / bp2837487
Plasmid length / bp32663266

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