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Team:Kyoto/CiC/Notebook/week1
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{{:Team:Kyoto/CiC/Notebook_tpl}} | {{:Team:Kyoto/CiC/Notebook_tpl}} | ||
| - | ==Week 1 : | + | ==Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide == |
{{:Team:Kyoto/Pad_start_dates}} | {{:Team:Kyoto/Pad_start_dates}} | ||
| - | ===Monday, | + | ===Monday, September 10=== |
====To Do on 24 July==== | ====To Do on 24 July==== | ||
| - | * | + | *PCR (HIV TAT LALAA peptide,11R LALLA peptide) |
| - | + | ||
*Electrophoresis | *Electrophoresis | ||
| - | + | *Restriction Enzyme Digestion | |
====Results==== | ====Results==== | ||
| - | ===== | + | =====Electrophoresis===== |
| - | *3-O-2 | + | [[Image:Kyoto_e_090724_1.png|250px]] |
| + | * Very clear bands were found. (See the gel image in right!) | ||
| + | * The bands are listed below. | ||
| + | |||
| + | {| border="1" style="text-align:center;width:50%" | ||
| + | |+Parts and Plasmid length | ||
| + | !!!3-O-2!!3-A-4 | ||
| + | |- | ||
| + | |Parts length / bp||2837||487 | ||
| + | |- | ||
| + | |Plasmid length / bp||3266||3266 | ||
| + | |} | ||
=====Digestion===== | =====Digestion===== | ||
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{{:Team:Kyoto/Pad_between_dates}} | {{:Team:Kyoto/Pad_between_dates}} | ||
| - | ===Tuseday, | + | |
| + | |||
| + | |||
| + | ===Tuseday, September 11=== | ||
====To Do on 24 July==== | ====To Do on 24 July==== | ||
| - | * | + | *Ligation(HIV TAT LALAA peptide,11R LALLA peptide) |
| - | * | + | *Transformation |
| - | + | ||
====Results==== | ====Results==== | ||
| - | ===== | + | =====Ligation===== |
| - | + | ||
| - | + | ||
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*Digested with EcoR1 / EcoR1 and Pst1 and run on a gel. | *Digested with EcoR1 / EcoR1 and Pst1 and run on a gel. | ||
Latest revision as of 18:43, 20 October 2009
- Home
- Cells in Cells
- Notebook
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
Week 1 : Construction of HIV TAT LALLA peptide and 11R LALLA peptide
Monday, September 10
To Do on 24 July
- PCR (HIV TAT LALAA peptide,11R LALLA peptide)
- Electrophoresis
- Restriction Enzyme Digestion
Results
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
| 3-O-2 | 3-A-4 | |
|---|---|---|
| Parts length / bp | 2837 | 487 |
| Plasmid length / bp | 3266 | 3266 |
Digestion
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 3-O-2 | Eco R1, PST1 |
| 2 | 3-A-4 | Eco R1, PST1 |
| 3 | 3-O-2 | Eco R1 |
| 4 | 3-A-4 | Eco R1 |
Tuseday, September 11
To Do on 24 July
- Ligation(HIV TAT LALAA peptide,11R LALLA peptide)
- Transformation
Results
Ligation
- Digested with EcoR1 / EcoR1 and Pst1 and run on a gel.
| NO. | Sample Name | Enzyme |
|---|---|---|
| 1 | 3-O-2 | Eco R1, PST1 |
| 2 | 3-A-4 | Eco R1, PST1 |
| 3 | 3-O-2 | Eco R1 |
| 4 | 3-A-4 | Eco R1 |
Electrophoresis
- Very clear bands were found. (See the gel image in right!)
- The bands are listed below.
| 3-O-2 | 3-A-4 | |
|---|---|---|
| Parts length / bp | 2837 | 487 |
| Plasmid length / bp | 3266 | 3266 |
