Team:Newcastle/IntroductoryLabwork/1 July 2009

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Introductory Lab Session: 1st July 2009

Today's introductory lab session saw the team progress with the next steps in the making competent cells procedure (i.e. steps 2 and 3 in the 'Pre-culture section). The team also looked at the future steps involved in the protocol and prepared solutions and equipment accordingly. This lab session (as well as the previous one) taught the team the importance of planning, organisation and time management.

Things needed for today's session

Things we need for today include:

• LB
• Containers
• Sterile tubes
• Space in orbital shaking incubator for tonight

Progressing with making competent cells

• We plated out single cultures from yesterday's plates for a practice.
• We inoculated 2ml of LB with single cultures from yesterday's plates and put them in the shaking incubator.
• We made up some 50% glycerol and it was sent to be autoclaved.

Preparations for next lab session

Considerations

• How much equipment (e.g. tubes) and solutions have we used today? Is there enough for Thursday's lab session?

Things we need for tomorrow (Thursday):

General

1. Sterile tubes
2. Pipette tips
3. Solutions

'Culture' Step (from making competent cells protocol)

1. Sterile flasks for culture (500ml)
2. Spectrophotometer and cuvettes (where)
3. Arrange space in shaking incubator

'Harvest cells' Step (from making competent cells protocol)

1. Centrifuge/ centrifuge vial (200ml?)
2. Ice bucket and ice
3. Glycerol
4. Sterile microfuge tubes
5. Liquid nitrogen

June M T W T F S S [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/1_June_2009&action=edit 1] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/2_June_2009&action=edit 2] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/3_June_2009&action=edit 3] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/4_June_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/5_June_2009&action=edit 5] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/6_June_2009&action=edit 6] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/7_June_2009&action=edit 7] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/8_June_2009&action=edit 8] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/9_June_2009&action=edit 9] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/10_June_2009&action=edit 10] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/11_June_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/12_June_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/13_June_2009&action=edit 13] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/14_June_2009&action=edit 14] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/15_June_2009&action=edit 15] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/16_June_2009&action=edit 16] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/17_June_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/18_June_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/19_June_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/20_June_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/21_June_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/22_June_2009&action=edit 22] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/23_June_2009&action=edit 23] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/24_June_2009&action=edit 24] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/25_June_2009&action=edit 25] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/26_June_2009&action=edit 26] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/27_June_2009&action=edit 27] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/28_June_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/29_June_2009&action=edit 29] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/30_June_2009 30]

July M T W T F S S     [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/1_July_2009 1] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/2_July_2009 2] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/3_July_2009 3] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/4_July_2009&action=edit 4] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/5_July_2009&action=edit 5] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/6_July_2009 6] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/7_July_2009 7] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/8_July_2009 8] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/9_July_2009 9] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/10_July_2009 10] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/11_July_2009&action=edit 11] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/12_July_2009&action=edit 12] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/13_July_2009&action=edit 13] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/14_July_2009 14] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/15_July_2009 15] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/16_July_2009 16] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/17_July_2009&action=edit 17] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/18_July_2009&action=edit 18] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/19_July_2009&action=edit 19] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/20_July_2009&action=edit 20] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/21_July_2009&action=edit 21] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/22_July_2009&action=edit 22] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/23_July_2009 23] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/24_July_2009 24] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/25_July_2009 25] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/26_July_2009&action=edit 26] [http://2009.igem.org/Team:Newcastle/IntroductoryLabwork/27_July_2009 27] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/28_July_2009&action=edit 28] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/29_July_2009&action=edit 29] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/30_July_2009&action=edit 30] [http://2009.igem.org/wiki/index.php?title=Team:Newcastle/IntroductoryLabwork/31_July_2009&action=edit 31]