Team:Newcastle/Labwork/18 September 2009

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Lab Work - 18/09/09

Stochastic switch team

Today we did midipreps of the ara transformation and digested 10ul to run on a gel (EcoRI + PstI) Two bands could be seen one for the backbone and one correctly sied for the insert (~200bp) so this midiprep can be taken forward in order to do the ara-sspb double clone.

Today we also made up some fresh DNA ladder as we have had problems with degradation.

  • 40ul of 500ug/ml promega lambda HindIII digest (20ug in total)
  • 280ul H2O
  • 80ul loading dye

=400ul which was aliquoted into 4 tubes; 3 put in the freezer 1 in the fridge.



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