Team:PKU Beijing/Parts Characterization/BBa K228004/Growth
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- | =='''Description'''== | + | ==='''Description'''=== |
It has been observed that salicylate as a inducer may decrease the growth rate of E. coli, but what the pattern and level of this decrease is unknown to us. We characterized the growth rate of E. coli through the method described in the [[Team:PKU_Beijing/Parts_Characterization/BBa_K228004|Part Main]] Page. We processed the data as the protocol mentioned in the [[Team:PKU_Beijing/Parts_Characterization/BBa_K228004/Transfer|Transfer Function]] page, demonstrating that the decrease level is significantly and positively correlated with the concentration of salicylate. | It has been observed that salicylate as a inducer may decrease the growth rate of E. coli, but what the pattern and level of this decrease is unknown to us. We characterized the growth rate of E. coli through the method described in the [[Team:PKU_Beijing/Parts_Characterization/BBa_K228004|Part Main]] Page. We processed the data as the protocol mentioned in the [[Team:PKU_Beijing/Parts_Characterization/BBa_K228004/Transfer|Transfer Function]] page, demonstrating that the decrease level is significantly and positively correlated with the concentration of salicylate. | ||
- | =='''Data'''== | + | ==='''Data'''=== |
[[Image:PKU_BBa_K228004_Od_new.png|600px]] | [[Image:PKU_BBa_K228004_Od_new.png|600px]] | ||
======Figure 1 - The relationship between growth rate and concentration of the inducer. We detected cell numbers in 20mins, 40mins, 60mins, 80mins, 100mins and 120mins, presented by corresponding points in this figure. Error bars shows the upper and lower limit of confidential interval. These curves reveal the decrease on growth rate in the presence of the inducer, salicylate molecule. We observed that at each time point of 20mins, 40mins, 60mins, 80mins, 100mins and 120mins, the decrease level is significantly and positively correlated with the input, the concentration of salicylate.====== | ======Figure 1 - The relationship between growth rate and concentration of the inducer. We detected cell numbers in 20mins, 40mins, 60mins, 80mins, 100mins and 120mins, presented by corresponding points in this figure. Error bars shows the upper and lower limit of confidential interval. These curves reveal the decrease on growth rate in the presence of the inducer, salicylate molecule. We observed that at each time point of 20mins, 40mins, 60mins, 80mins, 100mins and 120mins, the decrease level is significantly and positively correlated with the input, the concentration of salicylate.====== | ||
- | =='''Protocol'''== | + | ==='''Protocol'''=== |
1. We characterized the growth rate of E. coli through the same method as described in the [[Team:PKU_Beijing/Parts_Characterization/BBa_K228004/Transfer|Transfer Function]] page. The time between repeated samplings is 20 minutes. As the same, 6 replicates were measured for each concentration of salicylate, and the concentration 0 group is to measure fluorescent background. | 1. We characterized the growth rate of E. coli through the same method as described in the [[Team:PKU_Beijing/Parts_Characterization/BBa_K228004/Transfer|Transfer Function]] page. The time between repeated samplings is 20 minutes. As the same, 6 replicates were measured for each concentration of salicylate, and the concentration 0 group is to measure fluorescent background. |
Revision as of 00:53, 18 October 2009
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