Team:PKU Beijing/Parts Characterization/BBa K228004/Transfer
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For the purpose of characterization, we connected [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] downstream of the salicylate promoter to make a composite part, [http://partsregistry.org/Part:BBa_K228850 BBa_K228850]. This report system allowed us to use Microplate Reader to test the salicylate inducible promoter indirectly via the fluorescence output of [http://partsregistry.org/Part:BBa_E0840 BBa_E0840], while the input is salicylate solution at a gradient of concentration. | For the purpose of characterization, we connected [http://partsregistry.org/Part:BBa_E0840 BBa_E0840] downstream of the salicylate promoter to make a composite part, [http://partsregistry.org/Part:BBa_K228850 BBa_K228850]. This report system allowed us to use Microplate Reader to test the salicylate inducible promoter indirectly via the fluorescence output of [http://partsregistry.org/Part:BBa_E0840 BBa_E0840], while the input is salicylate solution at a gradient of concentration. | ||
- | =='''Data'''== | + | ==='''Data'''=== |
[[Image:PKU_BBa_K228004_120min.png|400px|center|thumb|Figure 1 - Transfer function of BBa_K228004. This transfer function is the 120 min time-slice from the surface shown in Figure 3 (highlighted as a heavy black line). The data points represent the mean of 6 individual measurements. The corresponding error bars represent the 95% confidence interval in the mean of the independent measurements. The solid black curve was calculated by fitting a simple Hill function to the experimental measurements. The Y axis is the value of fluorescence normalized by the OD600 value, and the X axis is the concentration of the inducer. ]] | [[Image:PKU_BBa_K228004_120min.png|400px|center|thumb|Figure 1 - Transfer function of BBa_K228004. This transfer function is the 120 min time-slice from the surface shown in Figure 3 (highlighted as a heavy black line). The data points represent the mean of 6 individual measurements. The corresponding error bars represent the 95% confidence interval in the mean of the independent measurements. The solid black curve was calculated by fitting a simple Hill function to the experimental measurements. The Y axis is the value of fluorescence normalized by the OD600 value, and the X axis is the concentration of the inducer. ]] | ||
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[[Image:PKU_BBa_K228004_All6_new.png|400px|center|thumb|Figure 2 – Transfer functions of BBa_K228004 in different time slices from the time and dose dependent input-output surface. The data points represent the mean of 6 individual measurements. The corresponding error bars denoting the 95% confidence interval in the mean of the independent measurements. These solid curves were calculated by fitting simple Hill functions to the experimental measurements. The Y axis is the value of fluorescence normalized by the OD600 value, and the X axis is the concentration of the inducer. As we can see, the level of induction triggered by the same concentration of inducer (higher than 10^-7 mol/L, causing concentration of 10^-10 mol/L has no significantly positive differences with the blank) will be enhanced as the induction time extended, so it is the same with the saturation. ]] | [[Image:PKU_BBa_K228004_All6_new.png|400px|center|thumb|Figure 2 – Transfer functions of BBa_K228004 in different time slices from the time and dose dependent input-output surface. The data points represent the mean of 6 individual measurements. The corresponding error bars denoting the 95% confidence interval in the mean of the independent measurements. These solid curves were calculated by fitting simple Hill functions to the experimental measurements. The Y axis is the value of fluorescence normalized by the OD600 value, and the X axis is the concentration of the inducer. As we can see, the level of induction triggered by the same concentration of inducer (higher than 10^-7 mol/L, causing concentration of 10^-10 mol/L has no significantly positive differences with the blank) will be enhanced as the induction time extended, so it is the same with the saturation. ]] | ||
- | =='''Protocol'''== | + | ==='''Protocol'''=== |
*'''Materials''' | *'''Materials''' |
Revision as of 00:49, 18 October 2009
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