Team:PKU Beijing/Parts Characterization/BBa K228004/Transfer
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==='''Data'''=== | ==='''Data'''=== | ||
- | [[Image:PKU_BBa_K228004_120min.png|400px|center|thumb|Figure 1 - Transfer function of BBa_K228004. This transfer function is the 120 min time-slice from the time and dose dependent input-output surface. The data points represent the mean of 6 individual measurements. The corresponding error bars represent the 95% confidence interval in the mean of the independent measurements. The solid black curve was calculated by fitting a simple Hill function to the experimental measurements. The Y axis | + | [[Image:PKU_BBa_K228004_120min.png|400px|center|thumb|Figure 1 - Transfer function of BBa_K228004. This transfer function is the 120 min time-slice from the time and dose dependent input-output surface. The data points represent the mean of 6 individual measurements. The corresponding error bars represent the 95% confidence interval in the mean of the independent measurements. The solid black curve was calculated by fitting a simple Hill function to the experimental measurements. The Y axis denotes the value of fluorescence normalized by the OD600 value, and the X axis denotes the concentration of the inducer. ]] |
- | [[Image:PKU_BBa_K228004_All6_new.png|400px|center|thumb|Figure 2 – Transfer functions of BBa_K228004 in different time slices from the time and dose dependent input-output surface. The data points represent the mean of 6 individual measurements. The corresponding error bars denoting the 95% confidence interval in the mean of the independent measurements. These solid curves were calculated by fitting simple Hill functions to the experimental measurements. The Y axis | + | [[Image:PKU_BBa_K228004_All6_new.png|400px|center|thumb|Figure 2 – Transfer functions of BBa_K228004 in different time slices from the time and dose dependent input-output surface. The data points represent the mean of 6 individual measurements. The corresponding error bars denoting the 95% confidence interval in the mean of the independent measurements. These solid curves were calculated by fitting simple Hill functions to the experimental measurements. The Y axis denotes the value of fluorescence normalized by the OD600 value, and the X axis denotes the concentration of the inducer. As we can see, the level of induction triggered by the same concentration of inducer (higher than 10^-7 mol/L, causing concentration of 10^-10 mol/L has no significantly positive differences with the blank) will be enhanced as the induction time being extended, so it is the same with the out-put saturation. ]] |
==='''Protocol'''=== | ==='''Protocol'''=== |
Revision as of 12:21, 19 October 2009
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