Team:UNICAMP-Brazil/Notebooks/August 11

From 2009.igem.org

(Difference between revisions)
m (→Yeastguard)
(→YeastGuard)
 
(7 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:UNICAMP-Brazil/inc_topo}}
{{:Team:UNICAMP-Brazil/inc_topo}}
{{:Team:UNICAMP-Brazil/inc calendar}}
{{:Team:UNICAMP-Brazil/inc calendar}}
 +
__NOTOC__
-
==Coliguard: Selection of transformants==
+
==''' ColiGuard '''==
-
Today we selected 4 colonies of each transformation to confirm it. We inocculated them separetaly in LB media containg the appropriate antibiotic and let them grow O/N.
+
====Selection of transformants====
-
==Yeastguard==
+
*<p style=”text-align:justify;”>Today we selected 4 colonies of each transformation to confirm it. We inoculated them separetely in LB medium containing the appropriate antibiotic and let them grow O/N.</p>
-
We decided that we won´t need biobrick BBa_J04630, because the other two reporters are ok for our constructions.
+
==''' YeastGuard '''==
-
As we need to get the DNA of BBa_J63002, we decided to repeat the plasmid extractions of the other five biobricks we are really going to use: BBa_J63005, BBa_J63003, BBa_E2030, BBa_E2020 and BBa_J63010.
+
====Recovering the biobricks====
-
In order to do the mini-prep tomorrow, we got the colonies from the plates (of the six biobricks) and put them to grow in liquid medium.  
+
* We decided that we won´t need biobrick BBa_J04630, because the other two reporters are OK for our constructions.
 +
 
 +
* We're going to need large amounts of DNA, so we inoculated colonies in liquid media in order to repeat the plasmid extractions of the six biobricks we are really going to use.
 +
 
 +
 
 +
''Taís''
-
'''Taís'''
 
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 00:34, 22 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

ColiGuard

Selection of transformants

  • Today we selected 4 colonies of each transformation to confirm it. We inoculated them separetely in LB medium containing the appropriate antibiotic and let them grow O/N.

YeastGuard

Recovering the biobricks

  • We decided that we won´t need biobrick BBa_J04630, because the other two reporters are OK for our constructions.
  • We're going to need large amounts of DNA, so we inoculated colonies in liquid media in order to repeat the plasmid extractions of the six biobricks we are really going to use.


Taís




Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/August_11"