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Team:UNICAMP-Brazil/Notebooks/August 15
From 2009.igem.org
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==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
| - | ====RBS and CFP | + | ====Biobricks recovered: RBS and CFP digestion==== |
| - | * Today we started the construction of our first biobrick. After | + | *<p style=”text-align:justify;”>Today we started the construction of our first biobrick. After quantification of the miniprep products (from August 11) we performed the digestion ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]) with ''Spe''I/''Pst''I and ''Xba''I/''Pst''I of plasmids containing the RBS and the CFP reporter respectively in order to connect these parts.</p> |
| - | * After the digestion we isolated the target bands from the agarose gel, according to | + | *<p style=”text-align:justify;”>After the digestion we isolated the target bands from the agarose gel, according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Purification_of_DNA_fragments_from_agarose_gels Protocol 7].</p> |
| - | * The CFP digested band was correctly purified from the gel, | + | [[image:20090815_Digestões-Lig 1.jpg|500px|center]] |
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| + | *<p style=”text-align:justify;”>The CFP digested band was correctly purified from the gel. Unfortunatly, we lost the RBS fragment. =( It must have been lost at some stage of the purification, therefore we could not ligate these two parts today.</p> | ||
''Raíssa'' | ''Raíssa'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 00:51, 22 October 2009
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