Team:UNICAMP-Brazil/Notebooks/August 17

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==''' YeastGuard '''==
==''' YeastGuard '''==
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====Trying to purify the RBS digested fragment====
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====Biobricks recovered: trying to purify the RBS digested fragment====
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* The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band was a little bigger than we expected, so we decided to check over it's size on Registry and we found that we ressuspended the wrong biobrick!!! Fortunately it's purification didn't work before! =)
+
* The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band was a little bigger than we expected , so we decided to check over it's size on Registry and we found that we ressuspended the wrong biobrick!!! Fortunately it's purification didn't work before! =)
* After this finding we checked the other biobricks and ressuspended the correct RBS. We transformed it in ''E. coli'', plated and grew it in liquid media.
* After this finding we checked the other biobricks and ressuspended the correct RBS. We transformed it in ''E. coli'', plated and grew it in liquid media.
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 +
[[Image:RBS.jpg‎|150px|center]]
''Raíssa''
''Raíssa''
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====Culture for Miniprep====
====Culture for Miniprep====
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* We apparently got the cells transformation; we made the cells selection and initiated the culture for tomorrow make the plasmid extraction.
+
*<p style=”text-align:justify;”>We apparently got the transformed cells; we made the cells selection and inoculated the culture in liquid medium for tomorrow's plasmid extraction.</p>
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+
-
====Recovering two RBSs====
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* We recovery two Biobricks
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BBa_J61100 RBS (Ribosome Binding Site Family Member)
+
====Recovering two RBS====
-
BBa_J61107 RBS (Ribosome Binding Site Family Member)
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*<p style=”text-align:justify;”>We recovered two biobricks:</p>
 +
- BBa_J61100 RBS (Ribosome Binding Site Family Member)
 +
- BBa_J61107 RBS (Ribosome Binding Site Family Member)
''Luige''
''Luige''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 01:00, 22 October 2009

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YeastGuard

Biobricks recovered: trying to purify the RBS digested fragment

  • The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band was a little bigger than we expected , so we decided to check over it's size on Registry and we found that we ressuspended the wrong biobrick!!! Fortunately it's purification didn't work before! =)
  • After this finding we checked the other biobricks and ressuspended the correct RBS. We transformed it in E. coli, plated and grew it in liquid media.
RBS.jpg

Raíssa

ColiGuard

Culture for Miniprep

  • We apparently got the transformed cells; we made the cells selection and inoculated the culture in liquid medium for tomorrow's plasmid extraction.

Recovering two RBS

  • We recovered two biobricks:

- BBa_J61100 RBS (Ribosome Binding Site Family Member)

- BBa_J61107 RBS (Ribosome Binding Site Family Member)

Luige




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