Team:UNICAMP-Brazil/Notebooks/August 17

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* After this finding we checked the other biobricks and ressuspended the correct RBS. We transformed it in ''E. coli'', plated and grew it in liquid media.
* After this finding we checked the other biobricks and ressuspended the correct RBS. We transformed it in ''E. coli'', plated and grew it in liquid media.
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[[Image:RBS.jpg‎ |center|‎]]
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[[Image:RBS.jpg‎ |center|100px‎]]
''Raíssa''
''Raíssa''

Revision as of 02:10, 21 October 2009

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YeastGuard

Biobricks recovered: trying to purify the RBS digested fragment

  • The RBS band purification performed on August 15 didn't work, so we digested the plasmid again. Curiously the digested band was a little bigger than we expected , so we decided to check over it's size on Registry and we found that we ressuspended the wrong biobrick!!! Fortunately it's purification didn't work before! =)
  • After this finding we checked the other biobricks and ressuspended the correct RBS. We transformed it in E. coli, plated and grew it in liquid media.
100px‎

Raíssa

ColiGuard

Culture for Miniprep

  • We apparently got the cells transformation; we made the cells selection and initiated the culture for tomorrow make the plasmid extraction.

Recovering two RBSs

  • We recovery two Biobricks

BBa_J61100 RBS (Ribosome Binding Site Family Member)

BBa_J61107 RBS (Ribosome Binding Site Family Member)


Luige




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