The biobrick BBa_J63002 was ressuspended from the plate and the transformed E. coli have occupied the whole plates, both LB-AMP and LB-KAN plates. We decided to replicate these colonies in other plates in order to get isolated colonies for the miniprep.
Taís
ColiGuard
Enzyme restriction
We make the miniprep and immediately make enzyme restriction with XbaI e PstI in order to prove the part existing in the plasmids. We didn’t find the correct band size (514 bp); we just found the linear plasmid size band.
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