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Team:UNICAMP-Brazil/Notebooks/August 19
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==''' YeastGuard '''== | ==''' YeastGuard '''== | ||
| - | ==== | + | ====Recovering the biobricks==== |
| - | In the LB-AMP plates we | + | *<p style=”text-align:justify;”>In the LB-AMP plates we managed to get isolated colonies, but the LB-KAN plates were the same as the first we had. We think that the antibiotic is not working on these LB-KAN plates, since the supposed antibiotic for the terminator vector is ampicilin.</p> |
| - | We selected colonies from the LB-AMP plates and inoculated in LB liquid media for the miniprep tomorrow. | + | |
| + | *<p style=”text-align:justify;”>We selected colonies from the LB-AMP plates and inoculated in LB liquid media for the miniprep tomorrow.</p> | ||
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| + | *<p style=”text-align:justify;”>We also inoculated colonies from the RBS plate for the miniprep.</p> | ||
''Taís'' | ''Taís'' | ||
| - | ==== | + | ==''' ColiGuard '''== |
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| + | ====Enzyme restriction==== | ||
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| + | *<p style=”text-align:justify;”>Today, we performed two enzymatic digestions of two RBS, recovered in order to prove the transformations. We got it!!! | ||
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| + | ''Luige'' | ||
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{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} | ||
Latest revision as of 01:10, 22 October 2009
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