"
Team:UNICAMP-Brazil/Notebooks/August 21
From 2009.igem.org
(Difference between revisions)
(→Miniprep, digestion and ligation of RBS) |
|||
| Line 8: | Line 8: | ||
====Miniprep, digestion and ligation of RBS==== | ====Miniprep, digestion and ligation of RBS==== | ||
| - | * Today we perfomed miniprep from the RBS transformed ''E. coli''. The fragment | + | * Today we perfomed miniprep from the RBS transformed ''E. coli''. The fragment size was correct and we decided to digest it without purifying. It worked. |
* After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol W. | * After the RBS plasmid digestion, we ligated it to CFP (the reporter gene) according to the Protocol W. | ||
Revision as of 19:01, 7 September 2009
| ||||||||||||||||||||||||||||||||||
