Team:UNICAMP-Brazil/Notebooks/August 23

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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' YeastGuard '''== '''Still fighting for RBS''' * We digested more plasmids with the ''Spe''I and...)
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'''Still fighting for RBS'''
'''Still fighting for RBS'''
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* We digested more plasmids with the ''Spe''I and ''Pst''I enzymes([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]). We left it digest a little longer and put less amount of DNA into the reaction. It seamed to work.
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* We digested more plasmids with the ''Spe''I and ''Pst''I enzymes([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Digestion_reaction Protocol 14]). We left it digest a little longer and put less amount of DNA into the reaction. This time it seamed to work.
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[[image:20090822_Rá_digestao 2 rbs 22_08_09.JPG|150px|center]]   
[[image:20090822_Rá_digestao 2 rbs 22_08_09.JPG|150px|center]]   
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''Raíssa''
''Raíssa''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 06:33, 21 October 2009

Topo l2.gif topo_r_igem.gif
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YeastGuard

Still fighting for RBS

  • We digested more plasmids with the SpeI and PstI enzymes(Protocol 14). We left it digest a little longer and put less amount of DNA into the reaction. This time it seamed to work.
20090822 Rá digestao 2 rbs 22 08 09.JPG

Raíssa