Team:UNICAMP-Brazil/Notebooks/August 27
From 2009.igem.org
(Difference between revisions)
(→Recovering biobrick: terminator) |
(→PCR with DNA from Kluyveromices lactis) |
||
Line 24: | Line 24: | ||
====PCR with DNA from ''Kluyveromices lactis''==== | ====PCR with DNA from ''Kluyveromices lactis''==== | ||
- | We did the PCR reactions to amplificate the ''K. lactis'' sequences. We made a 20µL standardt reaction, using 0,8µM of each primer. The annealing temperatures were: 59ºC for DLD promoter; 57ºC for JEN1 promoter; 53ºC for JEN1 gene. | + | We did the PCR reactions to amplificate the ''K. lactis'' sequences. We made a 20µL standardt reaction, using 0,8µM of each primer. The annealing temperatures were: 59ºC for DLD promoter; 57ºC for JEN1 promoter; 53ºC for JEN1 gene (agarose gel above). |
====Recovering biobrick: terminator==== | ====Recovering biobrick: terminator==== |
Revision as of 06:57, 21 October 2009
|