Team:UNICAMP-Brazil/Notebooks/August 27

From 2009.igem.org

(Difference between revisions)
(→Terminator biobrick)
(→MicroGuards)
 
(8 intermediate revisions not shown)
Line 8: Line 8:
====Unicamp-Brazil Team's meeting!====
====Unicamp-Brazil Team's meeting!====
-
Today we got together, talked about what we've done and what we have to do in the next 50 days!!  
+
*<p style=”text-align:justify;”>Today we got together, talked about what we've done and what we have to do in the next 50 days!!</p>
-
Lots of work!!
+
*<p style=”text-align:justify;”>Lots of work!!</p>
==== Primers ====
==== Primers ====
-
WE RECEIVED ALL THE PRIMERS! =]
 
-
 
-
==''' YeastGuard '''==
 
-
 
-
====RBS+CFP====
 
-
The colonies grew in the plates!
 
-
 
-
====Primers====
 
-
We have eluted all the Yeastguard project primers (Protocol X).
 
-
 
-
====PCR with DNA from ''Kluyveromices lactis''====
 
-
We did the PCR reactions to amplificated the ''K. lactis'' sequences. We made a 20µL reaction, using 0,8µM of each primer and following the program in Protocol Z.  The annealing temperatures were: 59ºC for DLD promoter; 57ºC for JEN1 promoter; 53ºC for JEN1 gene.
 
-
 
-
====Terminator biobrick ====
 
-
We plated the terminator transformation in LB without antibiotic, because we could´t find cells when we plated on LB+Amp or LB+Kan.
 
-
 
-
''Taís''
 
 +
*<p style=”text-align:justify;”>WE RECEIVED ALL THE PRIMERS! =]</p>
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Latest revision as of 01:46, 22 October 2009

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

MicroGuards

Unicamp-Brazil Team's meeting!

  • Today we got together, talked about what we've done and what we have to do in the next 50 days!!

  • Lots of work!!

Primers

  • WE RECEIVED ALL THE PRIMERS! =]



Retrieved from "http://2009.igem.org/Team:UNICAMP-Brazil/Notebooks/August_27"