Team:UNICAMP-Brazil/Notebooks/August 27

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==== Primers ====
==== Primers ====
WE RECEIVED ALL THE PRIMERS! =]
WE RECEIVED ALL THE PRIMERS! =]
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==''' YeastGuard '''==
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 +
====RBS+CFP====
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The colonies grew in the plates!
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====Primers====
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We have eluted all the Yeastguard project primers (Protocol X).
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====PCR with DNA from ''Kluyveromices lactis''====
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We did the PCR reactions to amplificated the ''K. lactis'' sequences. We made a 20µL reaction, using 0,8µM of each primer and following the program in Protocol Z.  The annealing temperatures were: 59ºC for DLD promoter; 57ºC for JEN1 promoter; 53ºC for JEN1 gene.
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====Terminator biobrick ====
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We plated the terminator transformation in LB whithout antibiotic, because whe can’t find cells when we plated on LB+Amp or LB+Kan.
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''Taís''
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{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 19:07, 7 September 2009

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MicroGuards

Unicamp-Brazil Team's meeting!

Today we got together, talked about what we've done and what we have to do in the next 50 days!!

Lots of work!!

Primers

WE RECEIVED ALL THE PRIMERS! =]

YeastGuard

RBS+CFP

The colonies grew in the plates!

Primers

We have eluted all the Yeastguard project primers (Protocol X).

PCR with DNA from Kluyveromices lactis

We did the PCR reactions to amplificated the K. lactis sequences. We made a 20µL reaction, using 0,8µM of each primer and following the program in Protocol Z. The annealing temperatures were: 59ºC for DLD promoter; 57ºC for JEN1 promoter; 53ºC for JEN1 gene.

Terminator biobrick

We plated the terminator transformation in LB whithout antibiotic, because whe can’t find cells when we plated on LB+Amp or LB+Kan.

Taís





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