Team:UNICAMP-Brazil/Notebooks/August 28

From 2009.igem.org

(Difference between revisions)
(Colony PCR)
(Terminator biobrick)
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====Terminator biobrick====
====Terminator biobrick====
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* =====Colony PCR=====
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* Colony PCR
The colony PCR was made to confirm the presence of terminator transformants, because  the antibiotic resistence is not working to any antibiotics.  
The colony PCR was made to confirm the presence of terminator transformants, because  the antibiotic resistence is not working to any antibiotics.  
We plated the the cells in LB, and picked some colonies with a toothpick, inoculate on a backup LB plate and put the toothpick on a tube with 20µl H2O, then, denature this cells 95°C, 5 minutes and heat shock on ice 5 minutes. Spin the tubes and use 1µl to make the PCR (see protocol).
We plated the the cells in LB, and picked some colonies with a toothpick, inoculate on a backup LB plate and put the toothpick on a tube with 20µl H2O, then, denature this cells 95°C, 5 minutes and heat shock on ice 5 minutes. Spin the tubes and use 1µl to make the PCR (see protocol).
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=====Preparing for Miniprep=====
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* Preparing for Miniprep
We also innoculated ten colonies transformed with the terminator biobrick to do the miniprep tomorrow.
We also innoculated ten colonies transformed with the terminator biobrick to do the miniprep tomorrow.

Revision as of 19:29, 7 September 2009

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YeastGuard

RBS+CFP

We did the permanent colonies.

Taís

Kluyveromices lactis genes

The PCR for the K. lactis genes worked very well! =] We got specific bands for the 3 sequences.

GEL PCR

Taís

Terminator biobrick

  • Colony PCR

The colony PCR was made to confirm the presence of terminator transformants, because the antibiotic resistence is not working to any antibiotics.

We plated the the cells in LB, and picked some colonies with a toothpick, inoculate on a backup LB plate and put the toothpick on a tube with 20µl H2O, then, denature this cells 95°C, 5 minutes and heat shock on ice 5 minutes. Spin the tubes and use 1µl to make the PCR (see protocol).

  • Preparing for Miniprep

We also innoculated ten colonies transformed with the terminator biobrick to do the miniprep tomorrow.

Wesley