Team:UNICAMP-Brazil/Notebooks/August 29

From 2009.igem.org

(Difference between revisions)
(RNA extraction)
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[[Image:chicken_run_4.jpg|right]]
[[Image:chicken_run_4.jpg|right]]
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=====RNA extraction=====
=====RNA extraction=====
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We used the Trizol protocol for the three tissues (Protocol 8).
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We used the Trizol protocol for the three tissues ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/RNA_isolation_from_%27Gallus_gallus%27_spleen%2C_blood_and_oviduct Protocol 8]).
We obtained pellets from all tissues! Hope their concentration and quality are ok! =]   
We obtained pellets from all tissues! Hope their concentration and quality are ok! =]   
   
   
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====Terminator Biobrick====
====Terminator Biobrick====
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Today we perfomed miniprep of the Terminator Biobrick transformed ''E. coli'', according to Protocol 2 (see protocols section).
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Today we perfomed miniprep of the Terminator Biobrick transformed ''E. coli'', according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Mini-Prep Protocol 2].
''Wesley''
''Wesley''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 22:55, 3 October 2009

Topo l2.gif topo_r_igem.gif
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YeastGuard

Lysozyme

Material

We found a farm that creates chicken for food production, and they have donated us the organs we needed to make the RNA extraction of Gallus gallus: the blood, the spleen and the oviduct.

Chicken run 4.jpg


RNA extraction

We used the Trizol protocol for the three tissues (Protocol 8). We obtained pellets from all tissues! Hope their concentration and quality are ok! =]

Gleidson, Raíssa, Taís and Sula

Terminator Biobrick

Today we perfomed miniprep of the Terminator Biobrick transformed E. coli, according to Protocol 2.

Wesley