Team:UNICAMP-Brazil/Notebooks/August 5

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(YeastGuard - Yeast DNA extraction)
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__NOTOC__
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==Preparation of electrocompetent ''E. coli''==
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==''' MicroGuards '''==
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Today we prepared electrocompetent ''E. coli'' to use in our transformations according to protocol 4 (see protocol section).
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====Preparation of electrocompetent ''E. coli''====
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'''Marcelo and Luige'''
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* Today we prepared electrocompetent ''E. coli'' to use in our transformations according to Protocol 4 (see protocols section).
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==YeastGuard: Yeast DNA extraction==
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''Marcelo and Luige''
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Today we performed DNA extration from yeast ''Kluyveromyces lactis'' according to protocol 5 (see protocol section). The agarose gel showed that the mini-prep was successful, but we need to treat the DNA with RNAse.
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==''' YeastGuard '''==
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==== Yeast DNA extraction ====
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* Today we performed DNA extration from yeast ''Kluyveromyces lactis'' according to Protocol 5 (see protocols section). The agarose gel showed that the mini-prep was successful, but we need to treat the DNA with RNAse.
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Revision as of 16:54, 7 September 2009

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MicroGuards

Preparation of electrocompetent E. coli

  • Today we prepared electrocompetent E. coli to use in our transformations according to Protocol 4 (see protocols section).

Marcelo and Luige

YeastGuard

Yeast DNA extraction

  • Today we performed DNA extration from yeast Kluyveromyces lactis according to Protocol 5 (see protocols section). The agarose gel showed that the mini-prep was successful, but we need to treat the DNA with RNAse.