Team:UNICAMP-Brazil/Notebooks/August 5

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====Preparation of electrocompetent ''E. coli''====
====Preparation of electrocompetent ''E. coli''====
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* Today we prepared electrocompetent ''E. coli'' to use in our transformations according to Protocol 4 (see protocols section).
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* Today we prepared electrocompetent ''E. coli'' to use in our transformations according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Preparation_of_electrocompetent_E._coli Protocol 4].
''Marcelo and Luige''
''Marcelo and Luige''
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==== Yeast DNA extraction ====
==== Yeast DNA extraction ====
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* Today we performed DNA extration from yeast ''Kluyveromyces lactis'' according to Protocol 5 (see protocols section). The agarose gel showed that the miniprep was successful, but we need to treat the DNA with RNAse.
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* Today we performed DNA extration from yeast ''Kluyveromyces lactis'' according to [https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/Yeast_DNA_Extraction Protocol 5]. The agarose gel showed that the miniprep was successful, but we need to treat the DNA with RNAse.
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

Revision as of 23:08, 3 October 2009

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MicroGuards

Preparation of electrocompetent E. coli

  • Today we prepared electrocompetent E. coli to use in our transformations according to Protocol 4.

Marcelo and Luige

YeastGuard

Yeast DNA extraction

  • Today we performed DNA extration from yeast Kluyveromyces lactis according to Protocol 5. The agarose gel showed that the miniprep was successful, but we need to treat the DNA with RNAse.




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