Team:UNICAMP-Brazil/Notebooks/August 7

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====Recovering the biobricks====
====Recovering the biobricks====
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*<p style=”text-align:justify;”>5 plates from yesterday transformation had colonies. The other 2 transformed bacteria were replated in two diferents antibiotics and in higher cell concentration, in the morning. At night one of these plates showed colonies.
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*<p style=”text-align:justify;”>5 plates from yesterday transformation had colonies. The other 2 transformed bacteria were replated into two diferent antibiotics and in higher cell concentration, in the morning. At night one of these plates showed colonies.</p>
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* We inoculated some of the grew colonies of each biobrick in liquid media to perform miniprep tomorrow.</p>
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*<p style=”text-align:justify;”>We inoculated some of the grown colonies of each biobrick in liquid media to perform miniprep tomorrow.</p>
''Raíssa and Wesley''
''Raíssa and Wesley''
{{:Team:UNICAMP-Brazil/inc_rodape}}
{{:Team:UNICAMP-Brazil/inc_rodape}}

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ColiGuard

Genomic DNA Extraction for Hly Operon Manipulation

  • We performed the genomic DNA Extraction from four pathogenic E. coli hly+ strains. We used the Aubusel 1998 protocol with modifications, Protocol 1.

Marcos

YeastGuard

Recovering the biobricks

  • 5 plates from yesterday transformation had colonies. The other 2 transformed bacteria were replated into two diferent antibiotics and in higher cell concentration, in the morning. At night one of these plates showed colonies.

  • We inoculated some of the grown colonies of each biobrick in liquid media to perform miniprep tomorrow.

Raíssa and Wesley




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