Team:UNICAMP-Brazil/Notebooks/August 7

(Difference between revisions)

Revision as of 05:35, 21 October 2009

We performed the genomic DNA Extraction from four pathogenic E. coli hly+ strains. We used the Aubusel 1998 protocol with modifications, Protocol 1.

Marcos

5 plates from yesterday transformation had colonies. The other 2 transformed bacteria were replated in two diferents antibiotics and in higher cell concentration, in the morning. At night one of these plates showed colonies.

<p style=”text-align:justify;”>We inoculated some of the grew colonies of each biobrick in liquid media to perform miniprep tomorrow.

Raíssa and Wesley

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 ====Recovering the biobricks====
-*<p style=”text-align:justify;”>5 plates from yesterday transformation had colonies. The other 2 transformed bacteria were re-plated in two diferents antibiotics and in higher cell concentration, in the morning. At night one of these plates showed colonies.  Overnight liquid cultures were grown from the single colonies that were transformed yesterday, each one contaning a diferent biobrick.</p>
+*<p style=”text-align:justify;”>5 plates from yesterday transformation had colonies. The other 2 transformed bacteria were replated in two diferents antibiotics and in higher cell concentration, in the morning. At night one of these plates showed colonies.
+*<p style=”text-align:justify;”>We inoculated some of the grew colonies of each biobrick in liquid media to perform miniprep tomorrow.</p>
 ''Raíssa and Wesley''
 {{:Team:UNICAMP-Brazil/inc_rodape}}