Team:UNICAMP-Brazil/Notebooks/August 7

(Difference between revisions)

Revision as of 16:57, 7 September 2009

We performed the genomic DNA Extraction from four pathogenic E. coli hly+ strains. We used the Aubusel 1998 protocol with modifications, Protocol 1 (see protocols section).

Marcos

5 plates from yesterday transformation had colonies. The other 2 transformed bacteria were re-plated in two diferents antibiotics and in higher cell concentration, in the morning. At night one of these plates showed colonies. Overnight liquid cultures were grown from the single colonies that were transformed yesterday, each one contaning a diferent biobrick.

Raíssa and Wesley

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 ====Genomic DNA Extraction for Hly Operon Manipulation====
-* We performed the genomic DNA Extraction from four pathogenic ''E. coli'' hly+ strains. We used the Aubusel 1998 protocol with modifications. The protocol is at the section "Protocols" (nº 1).
+* We performed the genomic DNA Extraction from four pathogenic ''E. coli'' hly+ strains. We used the Aubusel 1998 protocol with modifications, Protocol 1 (see protocols section).
 ''Marcos''