Team:UNICAMP-Brazil/Notebooks/July 24

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==''' ColiGuard '''==
==''' ColiGuard '''==
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==== R Plasmid Mini-Preps ====
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==== R Plasmid Minipreps ====
Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).
Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process).

Revision as of 18:27, 7 September 2009

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ColiGuard

R Plasmid Minipreps

Once we sucessfully recover the F plasmid, we now start the recovery procedure for the R plasmid. We will need this plasmid purified in order to isolate the FinO and FinP sequences (both related to the inhibition of the conjugative process). We performed mini-preps from R plasmid-containing cultures, according to Protocol 2 (see protocols section). As we did for F plasmid, we were also able to recover the R plasmid.

Gabriel, Marcelo and Victor.





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