Team:UNICAMP-Brazil/Notebooks/October 14

From 2009.igem.org

Revision as of 03:14, 21 October 2009 by Tsherig (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

YeastGuard

New strategy: pGEM

  • We did miniprep of 10 Adh1+Lysozyme colonies.

  • We digested the Adh1+Lysozyme/biofusion minipreps in order to connect it to the yeast expression vector (YEP). We used the XbaI and PstI enzymes. 9 of 10 digestions worked! =) The expected fragment size is 2000bp.

GEL

  • We chose two fragments and purified them from the agarose gel. We ligated them to YEP. We will transform it tomorrow.


Raíssa and Taís