Team:UNICAMP-Brazil/Notebooks/October 19

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*<p style=”text-align:justify;”>We did PCR with 30 yeast colonies transformed with Adh1+Lysozyme and pDLD+Lysozyme. Unfortunately none of the colonies had the correct vector =/</p>
*<p style=”text-align:justify;”>We did PCR with 30 yeast colonies transformed with Adh1+Lysozyme and pDLD+Lysozyme. Unfortunately none of the colonies had the correct vector =/</p>
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Revision as of 05:55, 21 October 2009

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YeastGuard

New strategy: pGEM

  • We sent the pDLD+lysozyme device to iGEM today. =)

Yeast experiments

  • There are plenty of yeast colonies in the pDLD+Lys and Adh1+Lys plates. =)

  • We did PCR with 30 yeast colonies transformed with Adh1+Lysozyme and pDLD+Lysozyme. Unfortunately none of the colonies had the correct vector =/

Adh1+lis2.jpg
PDLD+lis.jpg

  • We have no time to repeat the transformations =( Our hope is that there has been a problem to the PCR reaction and that the parts are correctly connected to the YEP vector. So we decided to go on with the transformed yeasts we have.

  • At night we find plenty yeast colonies in the pDLD+YFP and Adh1+YFP plates.

  • Considering our lack of time we decided to collect a pool of yeast colonies for each construction and inoculate in liquid media. This strategy was adopted for both constructions (pDLD+YFP and Adh1+YFP). These pre inocula will be used for induction tests of Lysozyme tomorrow.


Raíssa and Taís




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