Team:UNICAMP-Brazil/Notebooks/October 4

(Difference between revisions)

Revision as of 00:44, 21 October 2009

Yesterday's transformed and plated cells grew in the media! We found white and blue colonies in the plate just as expected (due to the beta-galactosidase gene contained in pGEM vector).
We selected 10 white colonies (those theorically contains our inserts in the correct position, since it interrupts the coding sequence of beta-galactosidase) from each plate and inoculated them into liquid LB-AMP media.
Inocula were keep at 37ºC, under 250 rpm, for an O/N period.

Fabi, Leo, Marcelo and Victor

We transformed the ligation made yesterday by eletroporation according to the Protocol 3

Léo

Only the plates containing E. coli transformed with pDLD and Lysozyme showed colonies. Tomorrow we will make colony PCR to collect the right ones.

Raíssa and Taís

@@ Line 8: / Line 8: @@
 ====finOP and Cre-Recombinase with pGEM strategy====
-* Yesterday's transformed and plated cells grew in the media! We found white and blue colonies in the plate just as expected (due to the beta-galactosidase gene contained in pGEM vector).
+*<p style=”text-align:justify;”>Yesterday's transformed and plated cells grew in the media! We found white and blue colonies in the plate just as expected (due to the beta-galactosidase gene contained in pGEM vector).</p>
-* We selected 10 white colonies (those theorically contains our inserts in the correct position, since it interrupts the coding sequence of beta-galactosidase) from each plate and inoculated them into liquid LB-AMP media.
+*<p style=”text-align:justify;”>We selected 10 white colonies (those theorically contains our inserts in the correct position, since it interrupts the coding sequence of beta-galactosidase) from each plate and inoculated them into liquid LB-AMP media.</p>
-* Inocula were keep at 37ºC, under 250 rpm, for an O/N period.
+*<p style=”text-align:justify;”>Inocula were keep at 37ºC, under 250 rpm, for an O/N period.</p>
 ''Fabi, Leo, Marcelo and Victor''