Team:UNICAMP-Brazil/Notebooks/September 13

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(New page: {{:Team:UNICAMP-Brazil/inc_topo}} {{:Team:UNICAMP-Brazil/inc calendar}} __NOTOC__ ==''' ColiGuard '''== ====finO+pSB1A3==== * After the O/N period, today we transformed the ligation fi...)
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* After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed protocol Z, without modifications (see Protocols section).
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* After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed protocol 3, without modifications (see Protocols section).
* We then plated the transfomed cells in LB-AMP media, and let them grow at 37ºC for an O/N period.
* We then plated the transfomed cells in LB-AMP media, and let them grow at 37ºC for an O/N period.

Revision as of 17:09, 16 September 2009

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ColiGuard

finO+pSB1A3

  • After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed protocol 3, without modifications (see Protocols section).
  • We then plated the transfomed cells in LB-AMP media, and let them grow at 37ºC for an O/N period.

finP+pSB1A3

  • We followed the same produce for the finP+pSB1A3 ligation.

Marcelo




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