After the O/N period, today we transformed the ligation finO+pSB1A3 into electrocompetent E. coli bacteria, strain DH10B. We followed protocol 3, without modifications (see Protocols section).
We then plated the transfomed cells in LB-AMP media, and let them grow at 37ºC for an O/N period.
finP+pSB1A3
We followed the same produce for the finP+pSB1A3 ligation.
Marcelo
YeastGuard
Lysozyme (orf), Jen1 (orf), DLD (promoter) and Jen1 (promoter)
We performed miniprep to get purified these parts (Protocol 2).
We then digest the plasmids obtained by the miniprep with XbaI (Protocol x).
The electrophoresis gel (down) showed nonspecific bands of al the parts digested.
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