Team:UNICAMP-Brazil/Notebooks/September 15
From 2009.igem.org
(→PY Promoter - PY + BBa_J23100 ligation) |
(→PY Promoter - PY + BBa_J23100 ligation) |
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*<p style=”text-align:justify;”>After the purification of BBa_J23100 from the gel we performed the SAP Dephosphorylation Protocol ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/SAP_Dephosphorylation Protocol 9]) to dephosphorylate the 5'-ends of the digested plasmid. It is necessary because the restriction enzymes XbaI and SpeI produces compatible cohesive ends, so the plasmid digested by this enzymes can religate. | *<p style=”text-align:justify;”>After the purification of BBa_J23100 from the gel we performed the SAP Dephosphorylation Protocol ([https://2009.igem.org/Team:UNICAMP-Brazil/Protocols/SAP_Dephosphorylation Protocol 9]) to dephosphorylate the 5'-ends of the digested plasmid. It is necessary because the restriction enzymes XbaI and SpeI produces compatible cohesive ends, so the plasmid digested by this enzymes can religate. | ||
- | *<p style=”text-align:justify;”>After the dephosphorylation we | + | *<p style=”text-align:justify;”>After the dephosphorylation we performed the ligation of PY1 + BBa_J23100 and PY2 + BBa_J23100 for an O/N period, according to protocol Y (see Protocols section). |
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''Fabiana and Leonardo'' | ''Fabiana and Leonardo'' | ||
{{:Team:UNICAMP-Brazil/inc_rodape}} | {{:Team:UNICAMP-Brazil/inc_rodape}} |
Revision as of 12:15, 19 October 2009
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