Team:UNICAMP-Brazil/Notebooks/September 2

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(→Lysozyme PCR)
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We did the PCR using the lysozyme primers. There was a total of 13 reactions: one for each organ, using the 2 cDNAs (1: pDN6; 2: oligoDT) and the RNA without treatment (because there was still genomic in it!).
We did the PCR using the lysozyme primers. There was a total of 13 reactions: one for each organ, using the 2 cDNAs (1: pDN6; 2: oligoDT) and the RNA without treatment (because there was still genomic in it!).
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[[image:20090902_PCR_Lysozyma_só_ela.jpg|250px|center]]   
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[[image:20090902_PCR_Lysozyma_só_ela.JPG|250px|center]]   
As you can see in the gel, we got specific bands from the oviduct sample! =]
As you can see in the gel, we got specific bands from the oviduct sample! =]

Revision as of 03:46, 21 October 2009

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YeastGuard

Lysozyme PCR

The results obtained today were extremely awaited. We did the PCR using the lysozyme primers. There was a total of 13 reactions: one for each organ, using the 2 cDNAs (1: pDN6; 2: oligoDT) and the RNA without treatment (because there was still genomic in it!).

20090902 PCR Lysozyma só ela.JPG

As you can see in the gel, we got specific bands from the oviduct sample! =]

Taís

RBS+CFP ligation

We verified using electrophoresis, the results of the digestion made yesterday (RBS+CFP plasmids). The gel showed we had no success with the digestions.

Wesley




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