Team:UNICAMP-Brazil/Notebooks/September 2

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YeastGuard

Lysozyme PCR

The results obtained today were extremely awaited. We did the PCR using the lysozyme primers. There was a total of 13 reactions: one for each organ, using the 2 cDNAs (1: pDN6; 2: oligoDT) and the RNA without treatment (because there was still genomic in it!).

GEL PCR

As you can see in the gel, we got specific bands from the oviduct sample! =]

Taís

RBS+CFP ligation

We verified using electrophoresis, the results of the digestion made yesterday (RBS+CFP plasmids). The gel showed we had no success with the digestions.

Wesley

@@ Line 9: / Line 9: @@
 The results obtained today were extremely awaited.
-We did the PCR using the lysozyme primers. There was a total of 13 reactions: one for each organ, using the 2 cDNAs and the RNA without treatment (because there was still genomic in it!).
+We did the PCR using the lysozyme primers. There was a total of 13 reactions: one for each organ, using the 2 cDNAs (1: pDN6; 2: oligoDT) and the RNA without treatment (because there was still genomic in it!).
 GEL PCR